Journal: The Plant Cell

Article Title: The R2R3-MYB Transcription Factors MYB14 and MYB15 Regulate Stilbene Biosynthesis in Vitis vinifera ^[W]

doi: 10.1105/tpc.113.117127

Figure Lengend Snippet: Expression of MYB14, MYB15, STSs, and Stilbene Accumulation during Grape Berry Development of V. vinifera cv Pinot Noir. (A) Columns represent MYB and STS transcript profiles in flower/skins. (B) Stilbene accumulation in flower/skins. (C) MYB and STS transcript profiles in seeds. (D) Stilbene accumulation in seeds. Data points are given as weeks from the onset of ripening (véraison; labeled with an arrow), with expression values as means of three replicate PCRs (n = 6) and error bars indicating se. Note that from 6 weeks before véraison (-6), berry skin has been separated from seeds. Flowering occurred 8 weeks before véraison. Accumulation of stilbenes was measured as methanol extracts of developing grape tissues using reverse-phase HPLC analysis. (B) and (D) show relative stilbene contents of trans-piceid in developing berries and in seeds as the mean of three different experiments, with error bars indicating se. Note that each time point during developmental series is a pool of >100 berries collected from ∼20 plants growing as described in Methods. FW, fresh weight.

Article Snippet: Analysis of Stilbenoids in Grapevine via HPLC HPLC analysis of stilbene compounds was performed on a reverse-phase HPLC device (Kontron Instruments; 322 pump system/360 autosampler/335 HPLC detector) with a Symmetry C18 column (3.5 μm, 4.6 mm × 150 mm [WAT200632]; Waters) protected by a guard column ( Czemmel et al., 2009 ).

Techniques: Expressing, Labeling

Journal: The Plant Cell

Article Title: The R2R3-MYB Transcription Factors MYB14 and MYB15 Regulate Stilbene Biosynthesis in Vitis vinifera ^[W]

doi: 10.1105/tpc.113.117127

Figure Lengend Snippet: Expression of MYB14, MYB15, STSs, and Stilbene Accumulation during Grape Berry Development of V. vinifera cv Pinot Noir. (A) Columns represent MYB and STS transcript profiles in flower/skins. (B) Stilbene accumulation in flower/skins. (C) MYB and STS transcript profiles in seeds. (D) Stilbene accumulation in seeds. Data points are given as weeks from the onset of ripening (véraison; labeled with an arrow), with expression values as means of three replicate PCRs (n = 6) and error bars indicating se. Note that from 6 weeks before véraison (-6), berry skin has been separated from seeds. Flowering occurred 8 weeks before véraison. Accumulation of stilbenes was measured as methanol extracts of developing grape tissues using reverse-phase HPLC analysis. (B) and (D) show relative stilbene contents of trans-piceid in developing berries and in seeds as the mean of three different experiments, with error bars indicating se. Note that each time point during developmental series is a pool of >100 berries collected from ∼20 plants growing as described in Methods. FW, fresh weight.

Article Snippet: HPLC analysis of stilbene compounds was performed on a reverse-phase HPLC device (Kontron Instruments; 322 pump system/360 autosampler/335 HPLC detector) with a Symmetry C18 column (3.5 μm, 4.6 mm × 150 mm [WAT200632]; Waters) protected by a guard column ( Czemmel et al., 2009 ).

Techniques: Expressing, Labeling

Journal: Frontiers in Plant Science

Article Title: Transcriptome-Wide Identification of Novel UV-B- and Light Modulated Flavonol Pathway Genes Controlled by VviMYBF1

doi: 10.3389/fpls.2017.01084

Figure Lengend Snippet: Selection of transgenic hairy root (HR) lines. Lines for microarray analysis were selected under the following criteria: flavonol accumulation as visualized by fluorescence microscopy on in situ diphenylboric acid-2- aminoethylester (DPBA) stained root cross sections (A) , flavonol content as shown by high performance liquid chromatography (HPLC) analysis (B) and gene expression analysis to verify transcript levels of the VviMYBF1 transgene and its well-characterized target VviFLS1 (C,D) . DBPA staining analysis shown in (A) was conducted with sections of the VviMYBF1 line 33 (left) and control line 77 (right). HPLC results in (B) are the mean values of three independent extractions from the same biological material. Gene expression analysis in (C,D) is shown relative to the expression of UBIQUITIN1 gene (previous TIGR database id: TC32075, VIT_16s0098g01190 ) with standard deviations derived from three qPCR runs with three technical replicates each. Stars indicate significant differences ( p < 0.05) between MYBF1 and control HRs based on t -test.

Article Snippet: High performance liquid chromatography analysis was performed on methanolic extracts of HR lines using a reverse phase HPLC (Kontron Instruments 322 pump system/360 autosampler/335 HPLC detector; Kontron) with a Symmetry C18 column (3,5 μm, 4,6 mm × 150 mm, catalog no. WAT200632, Waters) protected by a guard column ( ).

Techniques: Selection, Transgenic Assay, Microarray, Fluorescence, Microscopy, In Situ, Staining, High Performance Liquid Chromatography, Gene Expression, Control, Expressing, Derivative Assay